non-invasive cytology brush pcr for the diagnosis and causative species identification of american cutaneous leishmaniasis in peru非侵入性细胞学刷pcr诊断和病因物种鉴定的美国皮肤利什曼病在秘鲁.pdfVIP

Non-Invasive Cytology Brush PCR for the Diagnosis and Causative Species Identification of American Cutaneous Leishmaniasis in Peru 1. 1. 1 1 1,2 Braulio Mark Valencia , Nicolas Veland , Milena Alba , Vanessa Adaui , Jorge Arevalo , Donald E. Low3,4, Alejandro Llanos-Cuentas1,5, Andrea K. Boggild6,7* 1 Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia (UPCH), Lima, Peru, 2 Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Lima, Peru, 3 Public Health Laboratories, Public Health Ontario, Etobicoke, Ontario, Canada, 4 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada, 5 Hospital Nacional Cayetano Heredia, Lima, Peru, 6 Tropical Disease Unit, Division of Infectious Diseases, Toronto General Hospital, Toronto, Ontario, Canada, 7 Department of Medicine, University of Toronto, Toronto, Ontario, Canada Abstract Background: Traditional methods of detecting Leishmania from cutaneous lesions involve invasive diagnostic procedures, such as scrapings, which cause discomfort, require technical expertise, and carry risks of invasive procedures. We compared the performance of 2 novel, molecular-based non-invasive methods for the diagnosis of cutaneous leishmaniasis (CL). Methods: Consecutive patients presenting to the Leishmania Clinic at the Hospital Nacional Cayetano Heredia were enrolled. PCR was performed on filter paper lesion impressions (FPLIs), cytology brushes, and lancets for detection of Leishmania DNA. Smears from lesion scrapings and leishmanin skin test were also performed.