non-invasive in vivo imaging of calcium signaling in mice在小鼠体内非侵入性成像的钙信号.pdfVIP

Non-Invasive In Vivo Imaging of Calcium Signaling in Mice 1,2¤ 1 2 ¨ 2 1 1 2 Kelly L. Rogers , Sandrine Picaud , Emilie Roncali , Raphael Boisgard , Cesare Colasante , Jacques Stinnakre , Bertrand Tavitian , ˆ 1* Philippe Brulet ´ ´ ´ ´ 1 Unite d’Embryologie Moleculaire, CNRS URA 2578, Institut Pasteur, Paris, France, 2 CEA, Service Hospitalier Frederic Joliot, Inserm, U 803, Imagerie de ` l’expression des genes, Orsay, France Rapid and transient elevations of Ca2+ within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca2+ concentration ([Ca2+]) rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca2+-sensitive bioluminescent reporter GFP-aequorin targeted to the mitochondrial matrix were studied in several experimental paradigms. Rapid [Ca2+] rises inside the mitochondrial matrix could be readily detected during single-twitch muscle contractions. Whole body patterns of [Ca2+] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in mitochondrial [Ca2+] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole body recordings of newborn mice. This non-invasive imaging technique opens new avenues for the analysis of Ca2+ signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies. Citation: Rogers