noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography体内非侵入性,用光学相干断层扫描评估小鼠视网膜结构.pdfVIP

Noninvasive, In Vivo Assessment of Mouse Retinal Structure Using Optical Coherence Tomography 1. 1,2. 1 1 1 M. Dominik Fischer , Gesine Huber , Susanne C. Beck , Naoyuki Tanimoto , Regine Muehlfriedel , 1 3 3¤a ´ 3 4¤b Edda Fahl , Christian Grimm , Andreas Wenzel , Charlotte E. Reme , Serge A. van de Pavert , Jan 4 5 5 1 Wijnholds , Marek Pacal , Rod Bremner , Mathias W. Seeliger * 1 Division of Ocular Neurodegeneration, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tuebingen, Tuebingen, Germany, 2 Institute of Animal Welfare, Ethology and Animal Hygiene, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany, 3 Laboratory of Retinal Cell Biology, University of Zurich, Zurich, Switzerland, 4 Neuromedical Genetics, Netherlands Institute for Neuroscience, Amsterdam, The Netherlands, 5 Toronto Western Research Institute, University Health Network, Departments of Ophthalmology and Visual Science, and Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada Abstract Background: Optical coherence tomography (OCT) is a novel method of retinal in vivo imaging. In this study, we assessed the potential of OCT to yield histology-analogue sections in mouse models of retinal degeneration. Methodology/Principal Findings: We achieved to adapt a commercial 3rd generation OCT system to obtain and quantify high- resolution