non-metabolic membrane tubulation and permeability induced by bioactive peptidesnon-metabolic膜制管和生物活性肽引起的渗透率.pdfVIP

Non-Metabolic Membrane Tubulation and Permeability Induced by Bioactive Peptides ` 1,2 3 1,2 ´ 3 1,2 1,2 Antonin Lamaziere , Fabienne Burlina , Claude Wolf , Gerard Chassaing , Germain Trugnan , Jesus Ayala-Sanmartin * ´ ´ ´ 1 Institut National de la Sante et de la Recherche Medicale (INSERM), UMR 538, CHU Saint Antoine, Paris, France, 2 Universite Pierre et Marie Curie, ´ CHU Saint Antoine, Paris, France, 3 UMR Centre National de la Recherche Scientifique (CNRS) 7613, Universite Pierre et Marie Curie, Paris, France Background. Basic cell-penetrating peptides are potential vectors for therapeutic molecules and display antimicrobial activity. The peptide-membrane contact is the first step of the sequential processes leading to peptide internalization and cell activity. However, the molecular mechanisms involved in peptide-membrane interaction are not well understood and are frequently controversial. Herein, we compared the membrane activities of six basic peptides with different size, charge density and amphipaticity: Two cell-penetrating peptides (penetratin and R9), three amphipathic peptides and the neuromodulator substance P. Methodology/Principal Findings. Experiments of X ray diffraction, video-microscopy of giant vesicles, fluorescence spectroscopy, turbidimetry and calcein leakage from large vesicles are reported. Permeability and toxicity experiments were performed on cultured cells. The