non-native r1 substitution in the s4 domain uniquely alters kv4.3 channel gating非r1替换在s4域独特改变kv4.3通道控制.pdfVIP

Non-Native R1 Substitution in the S4 Domain Uniquely Alters Kv4.3 Channel Gating Matthew R. Skerritt, Donald L. Campbell* Department of Physiology Biophysics, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, New York, United States of America Abstract The S4 transmembrane domain in Shaker (Kv1) voltage-sensitive potassium channels has four basic residues (R1–R4) that are responsible for carrying the majority of gating charge. In Kv4 channels, however, R1 is replaced by a neutral valine at position 287. Among other differences, Kv4 channels display prominent closed state inactivation, a mechanism which is minimal in Shaker. To determine if the absence of R1 is responsible for important variation in gating characteristics between the two channel types, we introduced the V287R mutant into Kv4.3 and analyzed its effects on several voltage sensitive gating transitions. We found that the mutant increased the voltage sensitivity of steady-state activation and altered the kinetics of activation and deactivation processes. Although the kinetics of macroscopic inactivation were minimally affected, the characteristics of closed-state inactivation and recovery from open and closed inactivated states were significantly altered. The absence of R1 can only partially account for differences in the effective voltage sensitivity of gating between Shaker and Kv4.3. These results suggest that the S4 domain serves an important functional role in Kv4 channel activation and deactivation processes, and also those of closed-state inactivation and recovery. Citation: Skerritt MR, Campbell DL (2008) Non-Native R1 Substitution in the S4 Domain Uniquely Alters Kv4.3 Channel Gating. PLoS ONE 3(11): e3773. doi:10.1371/journal.pone.0003773 Editor: Arnold Schwartz, University of Cincinnati, United States of America