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Nonsex Genes in the Mating Type Locus of Candida albicans Play Roles in a/a Biofilm Formation, Including Impermeability and Fluconazole Resistance Thyagarajan Srikantha., Karla J. Daniels., Claude Pujol, Nidhi Sahni, Song Yi, David R. Soll* The Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa City, Iowa, United States of America Abstract The mating type locus (MTL) of Candida albicans contains the mating type genes and has, therefore, been assumed to play an exclusive role in the mating process. In mating-incompetent a/a cells, two of the mating type genes, MTLa1 and MTLa2, encode components of the a1-a2 corepressor that suppresses mating and switching. But the MTL locus of C. albicans also contains three apparently unrelated ‘‘nonsex’’ genes (NSGs), PIK, PAP and OBP, the first two essential for growth. Since it had been previously demonstrated that deleting either the a/a copy of the entire MTL locus, or either MTLa1 or MTLa2, affected virulence, we hypothesized that the NSGs in the MTL locus may also play a role in pathogenesis. Here by mutational analysis, it is demonstrated that both the mating type and nonsex genes in the MTL locus play roles in a/a biofilm formation, and that OBP is essential for impermeability and fluconazole resistance. Citation: Srikantha T, Daniels KJ, Pujol C, Sahni N, Yi S, et al. (2012) Nonsex Genes in the Mating Type Locus of Candida albicans Play Roles in a/a Biofilm Formation, Including Impermeability and Fluconazole Resistance. PLoS Pathog 8(1): e1002476. doi:10.1371/journal.ppat.1002476 Editor: Aaron P. Mitchell, Carnegie Mellon University, United States of America Received August 1, 2011; Accepted November 22, 2011; Published January 12, 2012 Copyright: 2012 Srikantha et al. This is an open-access article distributed under the terms of the Creative Commons Attributi