non-redundant role for il-12 and il-27 in modulating th2 polarization of carcinoembryonic antigen specific cd4 t cells from pancreatic cancer patients冗余作用的il - 12和il-27调制th2极化的癌胚抗原特异性cd4 t细胞从胰腺癌患者.pdfVIP

Non-Redundant Role for IL-12 and IL-27 in Modulating Th2 Polarization of Carcinoembryonic Antigen Specific CD4 T Cells from Pancreatic Cancer Patients 1,5 2,4,7 8 2,7 3,6 Elena Tassi , Marco Braga , Renato Longhi , Francesca Gavazzi , Giorgio Parmiani , Valerio Di Carlo2,4,7, Maria Pia Protti1,5* 1Tumor Immunology Unit, San Raffaele Scientific Institute, Milan, Italy, 2 Pancreas Unit, San Raffaele Scientific Institute, Milan, Italy, 3 Immuno-Biotherapy of Melanoma ` and Solid Tumors Unit, San Raffaele Scientific Institute, Milan, Italy, 4 Universita Vita-Salute San Raffaele, Milan, Italy, 5 Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy, 6 Division of Molecular Oncology, San Raffaele Scientific Institute, Milan, Italy, 7 Department of Surgery, San Raffaele Scientific Institute, Milan, Italy, 8 CNR-Istituto di Chimica del Riconoscimento Molecolare, Milan, Italy Abstract Background: Pancreatic cancer is a very aggressive disease with dismal prognosis; peculiar is the tumor microenvironment characterized by an extensive fibrotic stroma, which favors rapid tumor progression. We previously reported that pancreatic cancer patients have a selective Th2 skew in the anti-carcinoembryonic antigen (CEA) CD4+ T cell immunity, which correlates with the presence of a predominant GATA-3+ tumor lymphoid infiltrate. This has negative effects in both effective anti-tumor immunity and further favoring fibrinogenesis. Aim of this study was to evaluate whether the Th2 polarization of CEA-specific CD4+ T cells from pancreatic cancer patients is stable o