normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy正常的人类多能干细胞系表现出普遍的马赛克异倍性.pdfVIP

Normal Human Pluripotent Stem Cell Lines Exhibit Pervasive Mosaic Aneuploidy 1,2 1,3 4 5 1,3 Suzanne E. Peterson , Jurjen W. Westra , Stevens K. Rehen , Holly Young , Diane M. Bushman , 1 1,3 2 2 2 Christine M. Paczkowski , Yun C. Yung , Candace L. Lynch , Ha T. Tran , Kyle S. Nickey , Yu-Chieh 2 2,6 2 5 1 Wang , Louise C. Laurent , Jeanne F. Loring , Melissa K. Carpenter , Jerold Chun * 1 Department of Molecular Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America, 2 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, United States of America, 3 Biomedical Sciences Graduate Program, School of Medicine, University of California San Diego, San Diego, California, United States of America, 4 Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, 5 Robarts Research Institute, London, Ontario, Canada, 6 Department of Reproductive Medicine, University of California San Diego, San Diego, California, United States of America Abstract Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC – including induced pluripotent - lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome countin