nuclear importation of mariner transposases among eukaryotes motif requirements and homo-protein interactions核进口水手转座酶真核生物中主题需求和homo-protein交互.pdfVIP

Nuclear Importation of Mariner Transposases among Eukaryotes: Motif Requirements and Homo-Protein Interactions ´ 2 6 5 3 1,2 Marie-Veronique Demattei , Sabah Hedhili , Ludivine Sinzelle , Christophe Bressac , Sophie Casteret , ´4 2 2 5 6 1 Nathalie Moire , Jeanne Cambefort , Xavier Thomas , Nicolas Pollet , Pascal Gantet , Yves Bigot * 1 PRC, UMR INRA-CNRS 6175, Nouzilly, France, 2 GICC, UMR CNRS 6239, UFR des Sciences et Techniques, Tours, France, 3 IRBI, UMR CNRS 6035, UFR des Sciences et ´ Techniques, Tours, France, 4 UMR INRA 0483, UFR de Pharmacie, Tours, France, 5 Metamorphosys, CNRS UPS3201-Universite d’Evry Val d’Essonne, Genavenir 3 - Genopole ´ ´ Campus 1, Evry, France, 6 CIRAD, UMR 1098 Developpement et Amelioration des Plantes, Montpellier, France Abstract Mariner-like elements (MLEs) are widespread transposable elements in animal genomes. They have been divided into at least five sub-families with differing host ranges. We investigated whether the ability of transposases encoded by Mos1, Himar1 and Mcmar1 to be actively imported into nuclei varies between host belonging to different eukaryotic taxa. Our findings demonstrate that nuclear importation could restrict the host range of some MLEs in certain eukaryotic lineages, depending on their expression level. We then focused on the nuclear localization signal (NLS) in these proteins, and showed