riluzole-triggered gsh synthesis via activation of glutamate transporters to antagonize methylmercury-induced oxidative stress in rat cerebral cortexriluzole-triggered谷胱甘肽合成通过激活谷氨酸转运蛋白对抗methylmercury-induced氧化应激在老鼠大脑皮层.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2012, Article ID 534705, 12 pages doi:10.1155/2012/534705 Research Article Riluzole-Triggered GSH Synthesis via Activation of Glutamate Transporters to Antagonize Methylmercury-Induced Oxidative Stress in Rat Cerebral Cortex Yu Deng, Zhao-Fa Xu, Wei Liu, Bin Xu, Hai-Bo Yang, and Yan-Gang Wei Department of Enviromental Health, School of Public Health, China Medical University, Liaoning, Shenyang 110001, China Correspondence should be addressed to Zhao-Fa Xu, zfxu@ Received 10 May 2012; Revised 25 June 2012; Accepted 8 July 2012 Academic Editor: Luisa Minghetti Copyright © 2012 Yu Deng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Objective. This study was to evaluate the effect of riluzole on methylmercury- (MeHg-) induced oxidative stress, through promotion of glutathione (GSH) synthesis by activating of glutamate transporters (GluTs) in rat cerebral cortex. Methods. Eighty rats were randomly assigned to four groups, control group, riluzole alone group, MeHg alone group, and riluzole + MeHg group. The neurotoxicity of MeHg was observed by measuring mercury (Hg) absorption, pathological changes, and cell apoptosis of cortex. Oxidative stress was evaluated via determining reactive oxygen species (ROS), 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDAs), carbonyl, sulfydryl, and GSH in cortex. Glutamate (Glu) transport was studied by measuring Glu, glutamine (Gln), mRNA, and protein of glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1). Result. (1) MeHg induced Hg accumulation, patholo