stable plastid transformation for high-level recombinant protein expression promises and challenges稳定质体转变为高级重组蛋白表达的承诺和挑战.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 158232, 16 pages doi:10.1155/2012/158232 Review Article Stable Plastid Transformation for High-Level Recombinant Protein Expression: Promises and Challenges Meili Gao,1 Yongfei Li,2 Xiaochang Xue,3 Xianfeng Wang,4 and Jiangang Long5 1 Department of Biological Science and Engineering, Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China 2 School of Materials and Chemical Engineering, Xi’an Technological University, Xi’an, Shaanxi 710032, China 3 State Key Laboratory of Cancer Biology, Department of Biopharmaceutics School of Pharmacy, Fourth Military Medical University, Xi’an, Shaanxi 710032, China 4 Department of Anesthesiology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA 5 Institute of Mitochondrial Biology and Medicine, Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China Correspondence should be addressed to Jiangang Long, jglong@126.com Received 17 May 2012; Revised 10 August 2012; Accepted 24 August 2012 Academic Editor: Elvira Gonzalez De Mejia Copyright © 2012 Meili Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Plants are a promising expression system for the production of recombinant proteins. However, low protein productivity remains a major obstacle that limits extensive commercialization of whole plant and pl