the effect of different foam concentrations on sperm motility in japanese quail不同的泡沫浓度的影响在日本鹌鹑精子的运动性.pdfVIP

SAGE-Hindawi Access to Research Veterinary Medicine International Volume 2010, Article ID 564921, 4 pages doi:10.4061/2010/564921 Research Article The Effect of Different Foam Concentrations on Sperm Motility in Japanese Quail Avishek Biswas,1, 2 O. S. Ranganatha,1 and Jag Mohan1 1 Division of Physiology and Reproduction, Central Avian Research Institute, Izatnagar, Bareilly 243122, UP, India 2 Defence Institute of High Altitude Research, DRDO, C/o 56 APO, Leh 194101, JK, India Correspondence should be addressed to Avishek Biswas, drbiswas007@ Received 5 September 2010; Accepted 15 October 2010 Academic Editor: Timm C. Harder Copyright © 2010 Avishek Biswas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A study was conducted to determine the effect of foam extract on sperm motility in the male Japanese quail (Coturnix coturnix japonica ). Adult male quails (12 weeks) of heavy body weight strain were housed in individual cages and divided into 5 groups according to the size of their cloacal glands. The data indicated that the size of the cloacal gland was positively correlated with the frequency of foam secretion and total foam production. One gram of freshly collected clean foam was mixed with 1.0 mL of normal saline and homogenized for 10 minutes. After centrifugation at 35 000 rpm, the supernatant was used as 100% foam extract. The extract was diluted to 1:40, 1:20, 1:10, and 1:4 with normal saline to produce 2.5, 5.0, 10, and 25% foam extracts, respectively. ◦ ◦ 5% foam extract enhanced sperm survival at room temperatur