龙须菜琼胶多糖的提取纯化与性能表征.PDF

174 2007, Vol. 28, No. 08 食品科学 ※工艺技术 1,2,3 2 3 薛志欣 ，杨桂朋 ，王广策 (1.青岛大学化工学院，山东 青岛 266071； 2.中国海洋大学海洋化学理论与工程技术教育部重点实验室，山东 青岛　 266003； 3.中国科学院海洋研究所，山东 青岛 　266071) ：龙须菜(Gracilaria Lemaneiformis)的热水提取物，经DE-22和Sephadex G-200柱层析纯化，得到一种含微 量硫酸基的琼胶多糖，此多糖经 Q-Sepharose 柱层析鉴定为单一组分。紫外光谱显示它不含蛋白质、多肽及核酸， 红外光谱揭示它含3,6-内醚半乳糖特征吸收以及微量的硫酸基，热学性质表明在其0～700℃升温过程中存在两个失 重过程，旋光分析表明随温度的升高，该多糖水溶液存在一个由有序构象(螺旋结构)向无序构象(无规线团)转变的 过程 。 ：龙须菜；多糖；分离纯化；性能表征 Isolation, Purification and Characterization of Agar Polysaccharide from Gracilaria Lemaneiformis 1,2,3 2 3 XUE Zhi-xin ，YANG Gui-peng ，WANG Guang-ce (1.College of Chemical Engineering, Qingdao University, Qingdao 266071, China；2.Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266003, China； 3.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China) Abstract ：The crude polysaccharide was extracted from Gracilaria Lemaneiformis with hot water and was then purified by DE-22 and Sephadex G-200 column chromatograph. The purified polysaccharide contained little sulfate. Its homogeneity was identified with Q-Sepharose column chromatography. The ultraviolet spectrum showed that there are no protein and nucleic acids in the purified polysaccharide. The infrared spectrum indicated that there are special absorption of 3,6-anhydro-galactose and very little sulfate absorbance peaks. There are two weight loss courses during the increasing temperature from 0 to 700 ℃, while there i