洪永聪-茶树种质材料抗寒性早期鉴定评价体系的构建-090510.docVIP

茶树种质材料抗寒性早期鉴定评价体系的构建 洪永聪 王玉 丁兆堂* 张新富（青岛农业大学茶叶研究所，山东 青岛266109） 摘要：本文以表型性状优良的50份黄山群体种种质材料为研究对象，测定和分析它们的叶片解剖结构叶片生理指标和田间冻害发生。结果表明，栅栏组织/海绵组织（X1）为0.5，上表皮/海绵组织（X2）及栅栏组织/总厚度（X3）均为0.2，角质层/总厚度（X4）及海绵组织/总厚度（X5）均为0.05叶片解剖结构指数（Y）的计算公式为Y=0.5X1+0.2X2+0.2X3+0.05X4+0.05X5，50份种质材料被聚类成三个类群，并且可以预测第一、二类群中的30份材料具有较强的抗寒能力质膜相对透性（X1）茶树生理影响为0.45，可溶性糖含量（X2）为0.3，可溶性蛋白含量（X3）为0.25，得出叶片生理指数（Y）的计算公式为Y=0.45X1+0.3X2+0.25X3，30份种质材料被聚类成三个不同特点的类群，并且可以判断第一、二类群中的19份材料具有很强的抗寒能力叶片解剖结构指数叶片生理的评价体系，可应用于茶树幼苗期抗寒性的早期鉴定从而提高抗寒种质材料的筛选效率。 关键词：茶树种质；抗寒性中图分类号：： valuation system predicted on cold resistance of tea germplasm in young seedling HONG Yong-cong WANG Yu Ding Zhao-tang* ZHANG Xin-fu (Tea Research Institute, Qingdao Agriculture University, Qingdao 266109, Shandong, China ) Abstract: Anatomical structure, physiological index and frozen injury of leaves sampled from fifty specimens of tea germplasm, which had distinct phenotypic character and were selected from the species of Huangshan tea population, were analyzed. The results indicated that five ratio of those gemplasms varied significantly, including palisade tissue thickness to spongy tissue thickness (X1), upper epidermis thickness to spongy tissue thickness (X2), palisade tissue thickness to total thickness (X3), horny layer thickness to total thickness (X4), and spongy tissue thickness to total thickness (X5). The computing formula of anatomical structure index of leaves (Y1) was made to be Y1 = 0.5X1 + 0.2X2 + 0.2X3 + 0.05X4 + 0.05X5. Those germplasms could be clustered to three different groups, among which thirty germplasms belonged to the first group and the second group were predicted to have strong resistance to cold. Four indexes of those gemplasms were also tested to vary significantly, including relative permeability of plasma (X1), content of soluble sugar (X2), content of soluble protein (X3) and frozen injury. The computing formula of physiological index of leaves (Y2) was made to be Y2 = 0.45X1 + 0.3X2 + 0.25X3. Those germplasms could be clust