不同诱导子对枇杷悬浮细胞生长及熊果酸齐墩果酸含量的影响.PDF

2014,43(3): 187 ~ 191. Subtropical Plant Science 不同诱导子对枇杷悬浮细胞生长及熊果酸、 齐墩果酸含量的影响 李惠华，刘小英，徐 剑，常 强，王 伟，苏明华 （福建省亚热带植物研究所，福建省亚热带植物生理生化重点实验室，福建厦门 361006 ） 摘 要：在已建立的稳定枇杷细胞悬浮培养系的试验中，研究加入诱导子水杨酸（SA ）、茉莉酸甲酯（MJ ）、 水解酪蛋白（CH ）和酵母提取物（YE ）对枇杷悬浮细胞生长及次生代谢产物熊果酸（UA ）、齐墩果酸（OA ） -1 含量的影响。结果表明，从细胞生长、UA及OA含量三方面综合考虑，以MJ为 100.0 mg·L 时培养最佳，此时 细胞生长、UA及OA含量分别为对照的 1.081、2.540 及 2.590 倍。 关键词：枇杷；细胞悬浮培养；诱导子；熊果酸；齐墩果酸 Doi: 10.3969/j.issn.1009-7791.2014.03.001 中图分类号：Q943.1 文献标识码：A 文章编号：1009-7791(2014)03-0187-05 Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture LI Hui-hua, LIU Xiao-ying, XU Jian, CHANG Qiang, WANG Wei, SU Ming-hua (Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China) Abstract: Using the suitable loquat cell suspension culture as test materials, the influence of different elicitors (salicylic acid, SA; jasmonic acid, MJ; casein acid hydrolysate, CH; yeast extract, YE) on the cell growth, the accumulation of ursolic acid (UA) and Oleanic acid (OA) were studied. The -1 results showed that the addition of MJ 100.0 mg·L was advantageous to loquat cell suspension culture, the fresh weight, UA and OA content, were 1.081, 2.540, 2.590 times to the control, respectively. Key words: loquat; cell suspension; elicitor; ursolic acid; oleanic