STANDARD OPERATING PROCEDURE College （标准操作程序的大学）.pdfVIP

STANDARD OPERATING PROCEDURE JEOL JEM-2100 HR TRANSMISSION ELECTRON MICROSCOPE (TEM) EDS Chemical analysis Post column annular dark field detector Tomography unit for 3D images Cryogenic temperature control down to 77 K Point Resolution: 2.3 Å Line Resolution: 1.4 Å STEM resolution: 10 Å Magnetic imaging resolution: 22 Å Updated November 2014 1 Emergencies Power failure The microscope will shut down safely. When power is restored, it is necessary to restart the microscope manually (see below). Cooling Water Suspension The high voltage power and lens power will shut down, and the vacuum system will go into the protected state. Upon resumption of cooling water, the microscope must be restarted manually (see below). Emergency Stop Use the EM stop button (in left-hand cabinet) in case of fire or severe flood. Avoid this method of shutdown unless absolutely necessary. Updated November 2014 2 Start-Up Procedure Preliminaries Under normal circumstances, the microscope should be switched on and ready for use. Check to make sure that SF6 gas pressure 0.01 MPa (round dial gauge on left-hand unit behind microscope). Check to make sure that the air pressure is 0.4 MPa (top round dial gauge behind grill at base of column on left-hand side). Check to make sure that the gun gas pressure is 0.3 MPa (bottom round dial gauge behind grill at base of column on left-hand side). Check to make sure that the column pressure gauge on the sputter ion pump (SIP) power supply is 2.5 x 10-5 Pa (the rectangular dial gauge on the tall unit behind the microscope). M