祖辈（Ancestors）.docVIP

祖辈（Ancestors） First, the noun explanation 1. primary cultures (primary, Culture) were taken out of the tissues, inoculated and cultured for the first passage. The cell populations are heterogeneous and the cells are strongly interdependent. Cell independent survival was poor. 2. cloning rate (Cloning Efficiency): cells are diluted and dispersed into individual cells, forming percentage of small cells (clones) when cultured 3. cell lines (cell, line): primary culture cells after passage, cells called. 4. cells (Feeder): also known as trophoblast, fibroblasts or other cells into a single layer, after the use of large doses of radiation, so that cells lose proliferation ability, but can survive and metabolic activities. As a substrate, other cells are inoculated; the metabolism of the feed cell is also beneficial for other cells to grow. Feeder cells: special refractory cells, avoid the use of homologous cells. 5. anti progenitor (Atavism) cells lose phenomenon: tissue structure and cell morphology, differentiation weakened or not; to tend to a single cell, or gain eternal life, or become a group of cells with malignant traits. 6. contact inhibition (contact, inhibition): normal cells interact with each other to inhibit cell movement, a phenomenon known as inhibition (contact). 7. clonal cell lines: cells formed by single cells from a biologically identified cell line by single cell culture or by screening 8. subculture: when the cell proliferation reached a certain density, we need to isolate some cells and renew the culture medium, and continue to inoculate and cultivate. This process is called subculture. Otherwise, the cells will continue to survive. 9. stem cells: they have the ability to replicate themselves and can differentiate into functional cells under certain conditions. 10. cell division index (Mitotic, Index, MI): the number of divisions in each cell of a group of 1000 cells Two, Jane answer 1. what are the methods of separating cells? Separation of tissu