苏木伊红染色（Hematoxylin eosin staining）.docVIP

苏木伊红染色（Hematoxylin eosin staining） Hematoxylin and eosin staining, referred to as HE staining, is a routine staining method for histological techniques. Constant quality HE slice should be red and blue contrast, levels are clear shades. I. basic steps of hematoxylin eosin staining in paraffin sections: dewaxing (1) xylene I 15 minutes (2) xylene II (should be completely transparent) 10 minutes Gradually reduce the concentration of alcohol hydration (3) I (ethanol becomes opaque) 1-2 minutes (4) II absolute alcohol for 1-2 minutes (5) 95% alcohol for 1-2 minutes (6) 80% alcohol for 1-2 minutes (7) tap water wash for a while dyeing (8) distilled water for a moment (9) hematoxylin and eosin stained nuclei for 10 - 15 minutes (10) tap water wash for a while (11) 1% hydrochloric acid alcohol differentiation 0.5 - 1 minutes (12) flush water for a few hours (13) the solution of lithium carbonate is blue for 1 minutes (14) flush with water for 15 minutes to several hours (15) dye 0.5% Yi red water solution (contrast, dye) 2 - 5 minutes Gradually increase the concentration of alcohol dehydration (if dissolved in alcohol, red can direct 90% alcohol) (16) tap water to wash (differentiate Iraqi Red) for a while (17) 95% I alcohol for 1-2 minutes (18) 95% alcohol, II, I - 2 minutes (I9) anhydrous alcohol, I, 1 - 2 minutes (20) ethanol II L2 minutes transparent (21) xylene I 5 - 10 minutes (22) xylene II 5 - 10 minutes Transparency test: in the dark background, light is applied to the section. If there is a milky spot, the film is dehydrated Dehydration again. Sealing (23) under the coverslip sealed Results: the nuclei were blue, the cytoplasm was red, the red cells were orange, and the other components were red in color. Two. Heating during dyeing In order to shorten some degree of dyeing time, the method of heating is usually used. It is usually dipped or stained in the dye solution and placed in a temperature chamber of 37 or 56 DEG C to the desired time. Some need or close to