透射电子显微镜样品制备技术（Preparation technique of sample for transmission electron microscope）.docVIP

透射电子显微镜样品制备技术（Preparation technique of sample for transmission electron microscope） Preparation technique of sample for transmission electron microscope The basic requirements are as follows: keep the material structure and certain chemical composition as possible as possible; and the thickness of the material should not exceed 1000. Tissues and cells must be made into thin slices to obtain better resolution and sufficient contrast; using a variety of means, such as electronic staining, projection, etc. to improve the biological samples by negative staining electron scattering ability, in order to obtain better image contrast. The method of sample preparation varies according to the type of biomaterial and the purpose of the study. On biological tissues and cells, usually with ultramicrotomy, the large size materials appropriate to the size of the ultrathin section, and the use of electronic staining, and immunocytochemical labeling and autoradiography method showed various ultrastructure, various chemicals and change parts. For biological macromolecules (proteins, nucleic acids), bacteria, viruses and isolated organelles, granular materials, such as projection and negative staining, are used to improve the contrast and show the morphology and fine structure of particles. In addition, there are freezing fractures based on freeze fixation - freeze etching, freeze replacement, freeze drying and other technologies. Ultrathin sectioning involves small pieces of biological material soaked and embedded with liquid resin monomers and solidified into plastic blocks. They are then cut into ultrathin sections of 500 or about 50 by a microtome. The procedures for preparing ultrathin sections are similar to those of light microscopy, but the requirements of each step, and the reagents and methods used are very different. The choice of appropriate physical or chemical methods to kill tissue and cells rapidly, to maintain the normal structure of tissues and cells, and to minimize