酶标仪2（Microplate reader 2）.docVIP

酶标仪2（Microplate reader 2） Enzyme standard instrument, enzyme-linked immunosorbent assay (ELISA Reader), is a special instrument for enzyme-linked immunosorbent assay (ELISA). It can be simply divided into 2 categories: semi-automatic and automatic, but the working principle is basically the same. The core is a colorimeter that uses colorimetric methods to analyze antigen or antibody content. ELISA measurement generally requires that the final volume of the test solution is below 250ul and that the test can not be done with a general photoelectric colorimeter, so the photoelectric colorimeter in the microplate reader has special requirements. 6.1 enzyme linked immunosorbent assay ELISA test method I immunosorbent ELISA, is a marker technology, from the fluorescent antibody technique and isotope immune technology the development of a sensitive, specific, rapid and automation of modern technology. The basic principle of the method is the enzyme labeled antigen or antibody and enzyme crosslinking agent with enzyme labeled antigen or antibody, the enzyme labeled antigen or antibody can react specifically with solid carrier or tissue antigen or antibody, and firmly combined with the formation of immune complexes remain active. When the substrate is added, the substrate is catalyzed by enzymes to give a corresponding reactive color, and the color depth is proportional to the corresponding antigen or antibody content. Because this technique is based on antigen antibody reaction and enzyme catalysis, it has high sensitivity and specificity, and it is a powerful immunological test technique. The working principle and structure of 6.2 enzyme marker The microplate reader is in fact a special photoelectric colorimeter or spectrophotometer in disguise, and its basic working principle is basically the same as that of the photoelectric colorimeter. This is a single channel automatic sampling microplate working principle diagram, the light source light emitted by filter or monochrom