酶工程第6章（Enzyme engineering sixth chapters）.docVIP

酶工程第6章（Enzyme engineering sixth chapters） The sixth chapter is non-aqueous catalysis of enzymes The people in the past believed only in aqueous solution with enzyme catalytic activity. Study on the catalytic reaction of the enzyme in nonaqueous media: the nonaqueous medium in theory (including organic solvent, supercritical fluid, gas phase medium, ionic liquid medium), the structure and function of enzymes in non mechanism water medium of enzyme, enzymatic catalysis in nonaqueous media effect the dynamic aspects of the preliminary establishment of nonaqueous enzymology (non-aqueous Enzymology) theory system. The application of non-aqueous enzymatic catalysis, has achieved remarkable results. Research progress of 1. enzymes in nonaqueous catalysis The enzyme catalysis in nonaqueous media called non enzymatic catalysis. 1.1 enzyme catalysis in organic media: The enzyme catalysis in organic media refers to the catalytic reaction of enzyme in organic solvent containing a certain amount of water. It is suitable for both substrate and product or one of them for enzyme catalysis of hydrophobic substances. The enzyme in organic medium because of the space conformation to maintain the integrity of the structure and activity center, so to be able to play its catalytic function. The enzyme catalysis in organic media, enzyme substrate specificity and stereoselectivity and regioselectivity, bond selectivity and thermal stability have changed. 1.2 enzyme catalysis in gaseous medium: The enzyme catalytic gas phase medium is the enzyme catalyzed reactions in gas phase medium. It is applicable to the substrate or gas can be transformed into enzyme catalysis gases reaction. The gas medium due to low density, easy to spread, so the enzyme catalysis in the gas phase has different characteristics and catalysis in aqueous solution. Enzyme catalysis in 1.3 supercritical fluid medium: The enzyme catalysis in supercritical fluids refers to the enzyme catalytic reaction in supercritical flu