高中生物实验常用试剂（Common reagents for biology experiments in Senior High School）.docVIP

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高中生物实验常用试剂（Common reagents for biology experiments in Senior High School）.doc

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高中生物实验常用试剂（Common reagents for biology experiments in Senior High School）

高中生物实验常用试剂（Common reagents for biology experiments in Senior High School） Reagents commonly used in biology: 1.: Fehling reagent composition: 0.1g/ml NaOH (a liquid) and 0.05g/ml CuSO4 (solution B). Usage: apply a liquid Fehling reagent and the second liquid mixture, then the Fehling reagent after mixing into a liquid, such as water bath heating, to exist in the solution of reducing sugar, is a red brick. Class 2. sugar qualitative reagent: blue solution. Mixed with glucose, the boiling water bath will appear brick red precipitate. For the determination of glucose in urine. 3.: biuret reagent composition: 0.1g/ml NaOH (a liquid) and 0.01g/ml CuSO4 (solution B). Usage: to liquid 2ml is first added into a liquid, shake, addition of 3~4 drops of liquid ethylene, shake. If proteins are present in the test, they appear purple. 4. Sultan III: Usage: take Sultan III particles dissolved in 95% alcohol, shake well. Used to detect fat. Fat can be dyed orange (dyed red by Sultan IV). 5., two aniline: used to identify DNA. DNA is dyed blue when exposed to two aniline (boiling water bath). 6. methyl green: used to identify DNA. DNA is found to be blue-green when exposed to methyl green (at normal temperature). Piroxicam: tested for RNA, red 7, 50% alcohol solution: used to remove the floating color of Sultan III on fat. 8, 70% alcohol solution: used in medical clinical disinfection and sterilization. 9 and 95% alcohol solution: alcohol with a volume fraction of 95% can be used to agglutinate DNA 10, 15% hydrochloric acid, and 95% alcohol solution volume mixing, can be used to dissociate the root tip. 11. gentian violet solution or acetic acid magenta: basic dyes for chromosome staining, the former is deep blue, the latter is red Improved phenol fuchsin: detection of chromosome, red Janus Green: detects mitochondria, which makes mitochondria stained blue and green 12.20% of the liver, 3% of hydrogen peroxide, and 3.5% of ferric chloride were used to compare the catalytic efficien