一株海洋细菌对中肋骨条藻的溶解效应及其溶藻特性.pdfVIP

中国环境科学 2011,31(6) ：971~977 China Environmental Science 一株海洋细菌对中肋骨条藻的溶解效应及其溶藻特性 汪 辉,刘 玲,牛丹丹,刘兆普* (南京农业大学江苏省海洋生物学重点实验室,江苏 南京 210095) 摘要：从山东胶州湾分离得到 1 株海洋溶藻菌,暂将其命名为 JZ-1.根据生理生化及 16S rDNA 序列分析鉴定,菌株 JZ-1 属于交替单胞菌 属(Alteromonas).研究表明,菌株 JZ-1 对中肋骨条藻具有很好的溶解效果,它能够破坏藻细胞膜内物质的结构和细胞膜的完整性,使细胞膜 内物质流出导致藻细胞死亡.溶藻现象发生在细菌培养液的上清液和0.2µm 的过滤液中,而不是在细菌菌体中,这表明菌株JZ-1通过分泌代 谢物对中肋骨条藻产生溶解作用,且当菌株 JZ-1 由对数生长期向稳定期过渡时,其代谢物的溶藻率达到最大.代谢物分子量5kD,具有热稳 定性、耐酸性,但不耐碱. 关键词：海水富营养化；中肋骨条藻；溶藻细菌；溶藻机理 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2011)06-0971-07 Lysis effect and mechanisum of one bacterial strain on Skeletonema costatum. WANG Hui, LIU Ling, NIU Dan-dan, LIU Zhao-pu* (Key Laboratory of Applied Marine Biotechnology of Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China). China Environmental Science, 2011,31(6)：971~977 Abstract ：A strain of marine algae-lysing bacterium designated as JZ-1 was isolated from Jiaozhou Bay, China. Strain JZ-1 showed highly homology with Alteromonas sp. on the basis of morphological and biochemical characteristics and16S rDNA sequence analysis. Strain JZ-1 showed significant algicidal activity against Skeletonema costatum, it broke the materials structures in membrane and membrane integrity, and the materials leak through membrane to make the cells die. In addition, algicidal activity against S. costatum was detected in the filtrate and after filter through 0.2 µm syringe filter, not in the cells. Strain JZ-1 effects S. costatum by releasing certain algicidal substances, and this point was also confirmed by paper disk method. The metabolite had the highest algicidal activity at this moment when the bacterium transited to stationary phase from logarithmic growth phase. We tested some characteristics of the metabolite and the results were as follows: the molecular