花生MAPK基因的筛选和盐胁迫分析.pdfVIP

华北农学报 ·2017,32(3):27-32 花生MAPK 基因的筛选和盐胁迫分析 孔祥远,陈冠旭,秦贵龙,隋炯明,乔利仙,王晶珊,徐丽丽 (青岛农业大学 生命科学学院,山东省高校植物生物技术重点实验室,山东 青岛　 266109) 　 　 摘要:促有丝分裂原活化蛋白激酶(MAPK)在植物生长发育及抗逆胁迫过程中发挥重要作用。 为了解花生中 MAPK基因的情况,利用RNA测序技术对花生耐盐突变体(S2)和对照(S4)进行了转录组分析,筛选出了14个MAPK 基因,位于花生野生种基因组A组的6条染色体上。 聚类分析表明,花生14个MAPK基因中13个可以聚到已报道的 4个亚类中。 利用花生S2和S4构建了盐胁迫处理前后的表达谱,根据调整后的P值,筛选出6个MAPK 基因在S2 和(或)S4受盐胁迫诱导表达,分别属于A(1个)和D亚类(5 个)。 为花生MAPK 基因的功能验证和利用奠定了 基础。 关键词:花生;MAPK基因;盐胁迫;RNA测序 中图分类号:Q78;S565.03　 　 文献标识码:A　 　 文章编号:1000-7091(2017)03-0027-06 doi :10.7668/ hbnxb.2017.03.005 Screening and Salinity Stress Analysis ofMAPK Genes in Peanut KONG Xiangyuan,CHEN Guanxu,QIN Guilong,SUIJiongming, QIAO Lixian,WANGJingshan,XU Lili (College of Life Science,Qingdao Agricultural University,Key Lab of Plant Biotechnology in Universities of Shandong,Qingdao　 266109,China) Abstract:Mitogenactivatedproteinkinase (MAPK)playsanimportantroleinplantgrowthanddevelopment, and stress resistance in plant.In order to improve our understanding of the MAPK genes in peanut,RNA-seq was used to analyze one transcriptomic library constructed using a mutant with higher salinity resistance (S2) and its control (S4) in this study,14 MAPK geneswere screened and located on six chromosomes of A genome from wild species.Clustering analysisshowedthat 13outof 14MAPK geneswereassortedtofoursubgroupsreportedprevious- ly.Digital gene expressionprofileswereconstructedusing S2 and S4 samplesbeforeandafter salinity treatment,ac- cording to the adjustedP value,six MAPK geneswere found to be responsive to salinity treatment in S2 and (or) S4,which belonged to A (1)and D (5) subgroups,respectively.This study can provide the basis for functional i- dentification and application of MAPK genes in peanut. Key words:Peanut;MAPK gene;Salinity stress;RNA-se