不同容量液体复苏对内毒素性休克大鼠小肠基础电节律的影响 - 四川动物.doc

不同容量液体复苏对内毒素休克大鼠小肠基础电节律的影响 傅小云 李康 陈淼 喻田 [摘要]目的 探讨不同容量液体复苏对内毒素休克大鼠小肠基础电节律的影响。方法 48只SD大鼠，建立内毒素休克模型，根据液体复苏量随机分为：A、B、C三组，液体复苏速度分别为20ml/kg/h、30ml/kg/h、40ml/kg/h，维持目标血压（MAP）60-90mmHg ；D组：建立内毒素休克模型，不行液体复苏，以血管活性药物维持目标血压（MAP）60-90mmHg。观察比较各组休克前(T1)、休克后即刻（T2,建模成功后即刻）、休克后2h（T3）各组小肠基础电节律（小肠慢波电位频率、振幅、频率变异系数（%）振幅变异系数（%）]内毒素休克；液体复苏；小肠；基础电节律 The effect of different volumes of fluid resuscitation on small intestine basic electrical rhythm in rats with endotoxin-induced shock FU Xiaoyun1, LI Kang1,ChEN Miao1,YUTian2(1 The Department of critical care medicine of the Affiliated Hospital of Zunyi medical university;2 The Department of Anaesthesiology of Zunyi medical university, Guizhou Zunyi City 563003) [Abstract] Objective To investigate the effect of different volumes of fluid resuscitation on small intestine basic electrical rhythm in rats with endotoxin-induced shock. Methods 48 rats ,injection with endotoxin lipopolysaccharide to induce an endotoxin shock model, depending on the method of fluid resuscitation, were randomly divided into 4 groups:Group A,B,C(the speeds of fluid resuscitation were 20, 30, 40ml/kg/h respectively) and vasoactive agents were applied to maintain a target blood pressure ranged 60 to 90mmHg if necessary) and Group D (only vasoactive agents were applied to maintain a target blood pressure ranged 60 to 90mmHg). Changes in the coefficient of variation of the slow-wave amplitude (mV), frequency (%), amplitude (%) were measured in each group before shock(T1), immediately after shock (T2) and 2h after shock The ratio of intestinal dry-wet weight and pathological changes were examined at defined time points accordingly.Results The amplitude and frequency of intestinal slow-wave in all groups were decreased and the variation coefficients of slow-wave amplitude and frequency were increased significantly after endotoxin shock (p0.01);The amplitude and frequency of slow-wave and variation coefficients were improved significantly after fluid resuscitation (p0.05);T