MgSO_4对神经元NMDA兴奋毒性损伤的剂量依赖保护作用.pdfVIP

# 6# Journal of Clinical and Exp erim entalM edicine Vol. 7, N o. 10 Oct. 2008 MgSO NMDA 4 1 2 1 1 1 1 1 1 郁毅刚 蔡颖谦 王文浩 林俊明 黄巍 罗飞 李君 胡连水 ( 1解放军第一七五医院神 外科 福建 漳州 363000; 2 南方医科大学珠江医院全军神 医学研究所神 科 广东 广州 510282) 研究镁离子( Mg2+ ) 对神 细胞兴奋性氨基酸 N - 甲基- D - 天冬氨酸 ( NMDA ) 损伤的保护作用 2+ 采用原代培养的大鼠皮层神 细胞模型, 给予 NMDA 损伤, 加入不同浓度的 M g , 测定神 细胞损伤噻唑蓝 2+ ( MTT )变化 M g 对兴奋性氨基酸损伤保护作用在 1. 0~ 3. 0 mm ol/L 范围内存在, 2. 0 mmol/L效果最可靠 在重型颅脑损伤的临床治疗中应尽早使用镁剂, 剂量根据脑脊液浓度尽快调整到 2. 0 mm ol/L保护作用最好 NMDA 受体 镁离子(M g2+ ) 脑损伤 2+ 1 2 1 Protective effect ofMg on primary cortical damage in rats caused by NMDA. YU Yi- gang , CAI Ying - qian , WAN G W en - hao , et al. 1Depar tm ent of N eurosurg ery, The 175thH osp ital of P A, Zhangzhou Fuj ian 363000, China; 2Departm ent of N eurosurg ery, Zhuj iangH osp i tal, SouthenM edical University, Guangzhou Guangdong 510282, China. Abstract Objective To i vestigate the europrotective effect ofM g2+ for euro s dam aged by NMDA. M ethods By u si g prmi ary cultured rat cortical euro s, the cha ge i MTT of euro s damaged by NMDA a d protective effect ofM g2+ w ere observed. Results The europrotective effect ofM g2+ related to the co ce tratio ofMg2+ betw ee 1. 0 ~ 3. 0mm ol/L a d effective tmi e. 2. 0mmo l/L is the best co ce tratio for euro protective effect. Conclusion Mg2+ should be cli ically applied as early as possible, a d the dosage should be adju sted to 2. 0mmo l/L based o its co ce tratio i CSF. 2+ Key ords NMDA r; M g ; Brai i jury , 0. 25% 37! 30 m i , 1 m l , ,