原创力文档a non-invasive and real-time monitoring of the regulation of photosynthetic metabolism biosensor based on measurement of delayed fluorescence in vivo非侵入性和实时监测光合代谢调控的生物传感器基于延迟荧光测量体内.pdfVIP
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a non-invasive and real-time monitoring of the regulation of photosynthetic metabolism biosensor based on measurement of delayed fluorescence in vivo非侵入性和实时监测光合代谢调控的生物传感器基于延迟荧光测量体内
Sensors 2007, 7, 52-66
sensors
ISSN 1424-8220
© 2007 by MDPI
/sensors
Full Paper
A Non-invasive and Real-time Monitoring of the Regulation of
Photosynthetic Metabolism Biosensor Based on Measurement of
Delayed Fluorescence in Vivo
Lingrui Zhang, Da Xing * and Junsheng Wang
MOE Key Laboratory of Laser Life Science Institute of Laser Life Science, South China Normal
University, Guangzhou 510631, China
* Author to whom correspondence should be addressed. Telephone: +86-20-8521-0089 Fax: +86-20-
8521-6052; Email: xingda@ URL: /xingda.htm
Received: 12 November 2006 / Accepted: 17 January 2007 / Published: 24 January 2007
Abstract: In this paper, a new principle biosensor for non-invasive monitoring of the
regulation of photosynthetic metabolism based on quantitative measurement of delayed
fluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice as
excitation light source and a compact Single Photon Counting Module to collect DF signal,
is portable and can evaluate plant photosynthesis capacity in vivo. Compared with its
primary version in our previous report, the biosensor can better control environmental
factors. Moreover, the improved biosensor can automatically complete the measurements of
light and CO response curves of DF intensity. In the experimental study, the testing of the
2
improved biosensor has been made in soybean (Glycine max Zaoshu
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