a novel antioxidant phenyl disaccharide from populus tremula knotwood一种新型抗氧化苯二糖从杨树tremula knotwood.pdfVIP

Molecules, 2007, 12, 205-217 molecules ISSN 1420-3049 Full Paper A Novel Antioxidant Phenyl Disaccharide from Populus tremula Knotwood Madalina Neacsu 1,*, Vicente Micol 2, Laura Pérez-Fons 2, Stefan Willför 3,*, Bjarne Holmbom 3 2 and Ricardo Mallavia 1 Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine; Manastur 3-5, 400372 Cluj, Napoca, Romania 2 Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche 03202, Alicante, Spain; E-mails: vmicol@umh.es (Vicente Micol), laurapf@umh.es (Laura Pérez), r.mallavia@umh.es (Ricardo Mallavia) 3 Åbo Akademi University, Process Chemistry Centre, Porthansgatan 3, FI-20500 Turku/Åbo, Finland; E- mail: bholmbom@abo.fi (Bjarne Holmbom) * Authors to whom correspondence should be addressed: E-mails: neacsumro@, stefan.willfor@abo.fi; Phone (S. Willför): +358 2 215 4729, Fax +358 2 215 4868 Received: 8 January 2007; in revised form: 15 February 2007 / Accepted: 18 February 2007 / Published: 19 February 2007 Abstract: The complete characterization of two compounds obtained from the acetone extract of Populus tremula knotwood has been was achieved using LC-DAD-MS, MS/MS, IR and NMR. The new compounds were unequivocally identified as a mixture of the ester isomers of the (E) and (Z) p-coumarate of 1-O-rutinose. The isomers showed the capacity to inhibit lipid peroxidation induced by tert-butylhydroperoxide and to trap peroxyl radicals, as determined by a chemiluminescence method. These new phenyl disaccharides also showed a signifi