a putative role for amino acid permeases in sink-source communication of barley tissues uncovered by rna-seq假定的角色氨基酸通透酶在sink-source通信大麦rna-seq组织发现.pdfVIP

Kohl et al. BMC Plant Biology 2012, 12:154 /1471-2229/12/154 RESEARCH ARTICLE Open Access A putative role for amino acid permeases in sink-source communication of barley tissues uncovered by RNA-seq 1*† 2† 1 1 1 Stefan Kohl , Julien Hollmann , Frank R Blattner , Volodymyr Radchuk , Franka Andersch , 1 1 1 2 1 1* Burkhard Steuernagel , Thomas Schmutzer , Uwe Scholz , Karin Krupinska , Hans Weber and Winfriede Weschke Abstract Background: The majority of nitrogen accumulating in cereal grains originates from proteins remobilised from vegetative organs. However, interactions between grain filling and remobilisation are poorly understood. We used transcriptome large-scale pyrosequencing of flag leaves, glumes and developing grains to identify cysteine peptidase and N transporter genes playing a role in remobilisation and accumulation of nitrogen in barley. Results: Combination of already known and newly derived sequence information reduced redundancy, increased contig length and identified new members of cysteine peptidase and N transporter gene families. The dataset for N transporter genes was aligned with N transporter amino acid sequences of rice and Arabidopsis derived from Aramemnon database. 57 AAT, 45 NRT1/PTR and 22 OPT unigenes identified by this approach cluster to defined subgroups in the respective phylogenetic trees, among them 25 AAT, 8 NRT1/PTR and 5 OPT full-length sequences. Besides, 59 unigenes encoding cysteine peptidases were identified and subdivided into different families of the papain cysteine peptidase clade. Expression profiling