administration of hydrogen sulfide via extracorporeal membrane lung ventilation in sheep with partial cardiopulmonary bypass perfusion a proof of concept study on metabolic and vasomotor effects硫化氢管理局通过体外膜肺通气与部分羊心肺旁路灌注概念验证研究代谢和血管舒缩性的影响.pdfVIP

Derwall et al. Critical Care 2011, 15:R51 /content/15/1/R51 RESEARCH Open Access Administration of hydrogen sulfide via extracorporeal membrane lung ventilation in sheep with partial cardiopulmonary bypass perfusion: a proof of concept study on metabolic and vasomotor effects 1,2*† 1† 1 1 1 1 Matthias Derwall , Roland CE Francis , Kotaro Kida , Masahiko Bougaki , Ettore Crimi , Christophe Adrie , Warren M Zapol1, Fumito Ichinose1 Abstract Introduction: Although inhalation of 80 parts per million (ppm) of hydrogen sulfide (H S) reduces metabolism in 2 mice, doses higher than 200 ppm of H2S were required to depress metabolism in rats. We therefore hypothesized that higher concentrations of H2S are required to reduce metabolism in larger mammals and humans. To avoid the potential pulmonary toxicity of H S inhalation at high concentrations, we investigated whether administering H S 2 2 via ventilation of an extracorporeal membrane lung (ECML) would provide means to manipulate the metabolic rate in sheep. Methods: A partial venoarterial cardiopulmonary bypass was established in anesthetized, ventilated (fraction of inspired oxygen = 0.5) sheep. The ECML was alternately ventilated with air or air containing 100, 200, or 300 ppm  H2S for intervals of 1 hour. Metabolic rate was estimated on the basis of total CO2