fasudil and hemodynamic function in acute myocardial infarction myocardial tissue of bc1 2 and bax expression（fasudil和血流动力学功能在急性心肌梗死心肌组织的群体bc1 2和伯灵顿的表情）.docVIP

Fasudil and hemodynamic function in acute myocardial infarction myocardial tissue of Bc1 2 and Bax expression Author: Pannuo Na Tan Lijuan Ma Jian [Abstract] Objective To investigate the fasudil postoperative acute myocardial infarction (AMI myocardial tissue of Bc1 2, Bax expression in hemodynamic function. Methods Male Wistar rats by ligation of the left anterior descending artery production of AMI model, 24 h, survival of the rats were randomly divided into treatment group and AMI group. another randomly selected 10 rats were used as sham operation group, only threading in the left anterior descending artery was not ligated. treatment group received fasudil 5 mg / kg of intraperitoneal injection, 2 times a day, in AMI group and sham operation group was given the same amount of saline. were measured hemodynamics of left ventricular systolic pressure (LVSP, left ventricular end diastolic pressure (LVEDP, the maximum rise of left ventricular pressure and fall rate (+ dp / dt max, -dp/dtmax; by RT PCR method for the determination of Rho-kinase mRNA expression, immunohistochemical method determination of the changes in apoptosis related proteins Bcl 2 and Bax. the results of the AMI group, hemodynamic parameters myocardial tissue of Rho kinase mRNA, apoptosis related protein Bcl-2 and Bax expression compared with the sham group, the differences were significant (F = 14.79 ~ 178.79, q = 4.85 ~ 24.95, P lt;0.01 compared with the AMI group hemodynamic parameters of the treatment group improved myocardial tissue decreased expression of Rho kinase mRNA, apoptosis-related protein Bcl 2 expression was increased, the expression of Bax reduced, the differences were significant (q = 3.20 ~ 15.11, P lt;0.05, 0.01. Conclusions Rho kinase involved in the progress of cardiac myocyte apoptosis and heart failure after AMI, fasudil reduced apoptosis by inhibition of Rho kinase expression, protection of ischemic myocardium, slow the heart failure process. [Keywords:] myoca