C促红细胞生成素在脑梗死后微循环再通中的作用.docVIP

C促红细胞生成素在脑梗死后微循环再通中的作用 张起顺，陈 勇，王朝辉，吴春芳，赵 俊(河南大学淮河医院神经内科，河南省开封市 475000) 引用本文：张起顺，陈勇，王朝辉，吴春芳，赵俊. C促红细胞生成素在脑梗死后微循环再通中的作用[J].中国组织工程研究，2017，21():2534-2539. DOI:10.3969/j.issn.2095-4344.2017.16.013 ORCID: 0000-0002-9202-6093(张起顺) 文章快速阅读： 文题释义： 促红细胞生成素：微循环：Carbamylated erythropoietin promotes vascular microcirculation following cerebral infarction Zhang Qi-shun, Chen Yong, Wang Zhao-hui, Wu Chun-fang, Zhao Jun (Department of Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, China) Abstract BACKGROUND: Carbamylated erythropoietin (CEPO) cannot only remarkably promote the prognosis of cerebral infraction, but also improve the microcirculation. OBJECTIVE: To explore the underlying mechanism of CEPO promoting the microcirculation following cerebral infraction. METHODS: 150 Wistar rats were selected, and 120 rats were used for establishing the models of cerebral infarction, followed by allotted into four groups. The model rats were treated with 500, 1 000 and 2 000 u/kg CEPO as experimental groups, and those received no treatment as model group. The other 30 rats were as controls. Vascular endothelial cells were isolated and cultured in vitro, and the cell proliferation was detected by cell counting kit-8 assay. The expression levels of proliferation-related genes (Ki67 and p16) and vascular endothelial growth factor (VEGF) were detected using western blot assay. After selective silencing of VEGF through RNA interference, all above indicators were detected again. RESULTS AND CONCLUSION: Cell counting kit-8 assay results showed that the proliferation ability of vascular endothelial cells was increased with CEPO concentration increasing. Western blot assay results showed a significant upregulation of Ki67, p16 and VEGF. After shRNA-VEFG interference, these indicators had no positive correlation with the increased concentration of CEPO. Our findings indicate that CEPO can improve the proliferation of vascular endothelial cells in an ani