TLR4在实验性脑出血小鼠脑组织表达变化及其与神经功能缺损的相关性.docVIP

TLR4在实验性脑出血小鼠脑组织表达变化及其与神经功能缺损的相关性 钟琪，杨清武*，李俊祺，李敬诚，向静，王景周（第三军医大学大坪医院神经内科，重庆，400042） 摘要：目的 探讨Toll样受体4（Toll-like receptor 4，TLR4）在脑出血小鼠血肿周围脑组织中的表达变化及其与神经功能缺损的相关性。 方法 采用自体尾动脉血注入法复制脑出血小鼠模型,将30只雄性C57BL小鼠按照区组随机分组法分为假手术组、模型组，造模完毕后采取同样随机分组法根据不同时间点(术后6h、24h、48h、72 h，5 d)分为5个亚组，每组3只。各组于不同时间点取材，采用荧光定量多聚酶链反应法（real-time PCR）检测小鼠脑组织TLR4 mRNA的表达，同时测定神经功能缺损评分，并分析二者间的相关性。结果 两组组各时间点TLR4 mRNA均表达；与假手术组相比模型组TLR4 mRNA表达显著升高(P 0. 05)，且在72 h达高峰。模型组相同时间点TLR4 mRNA的表达与假手术组比较均明显增高，差异有统计学意义( P 0. 05)。脑出血后神经功能缺损明显加重( P 0.05)，以48－72 h为著。相关分析显示，TLR4的表达与神经功能缺损呈直线相关( R2=0.973,P 0.05)。 结论 结果提示TLR4在脑出血后脑损伤中具有重要作用，可能是脑出血后炎症反应的关键启动环节。 关键词：脑出血；炎症反应；TLR4 中图法分类号：R392.13;R394-33;R394.3 文献识别码：A Upregulated expression of Toll- like receptor 4 in brain tissue surrounding hematoma correlates with neurologic deficits in experimental cerebral hemorrhage mice Zhong Qi，Yang Qing-wu*，Li Jun-qi，Li Jing-cheng，Xiang Jing，Wang Jing-zhou (Department of Neurology，DaPing Hospital，Third Military Medical University，Chongqing 400042，China) Abstract：Objective To explore the expression of Toll-like receptor4 (TLR4) in brain tissue surrounding hematoma of cerebral hemorrhage mice and the correlation between Toll-like receptor4 and neurologic deficits. Methods The cerebral hemorrhage models of mice were made by injecting autologous arterial blood collected from caudal artery. Thirty male C57BL mice were randomly divided into 2 groups: sham-operation and model groups. The expression of TLR4 mRNA in the brain tissue surrounding hematoma was determined simultaneously by real-time PCR, simultaneous determination neurologic deficits, and to analyze pairwise correlation. Results The expression of TLR4 gene in model group was higher evidently than that in sham-operation group ( P 0. 05).Cerebral edema and neurologic deficits significantly increased,especially at 48-72th hour. Correlation analysis showed that the expression of TLR4 and cerebral edema and neurologic deficits were obviously positive correla