comparisons of sampling procedures and time of sampling for the detection of salmonella in danish infected chicken flocks raised in floor systems比较取样程序和时间的抽样检测沙门氏菌的丹麦感染鸡群生长在地面系统.pdfVIP

Acta vet. scand. 2002, 43, 21-30. Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems 1 2 1 By K. O. Gradel , J. Andersen and M. Madsen 1Department of Poultry, Fish and Fur-bearing Animals, Aarhus, 2Danish Zoonosis Centre, Copenhagen and Dan- ish Veterinary Institute, Denmark. Gradel KO, Andersen J, Madsen M: Comparisons of sampling and time of sam- pling for the detection of Salmonella in Danish infected chicken flocks raised in floor systems. Acta vet. scand. 2002, 43, 21-30. – Bacteriological follow-up samples were taken from 41 chicken (Gallus gallus) flocks in floor systems, where Salmonella enterica (Salmonella) had been detected either directly in bacteriological samples or in- directly by serological samples. Three types of follow-up samples were compared to each other within each flock: 1) 5 pairs of socks, analysed as 5 samples, 2) 2 pairs of socks, analysed as one sample, and 3) 60 faecal samples, analysed as one pooled sam- ple. Agreement between sampling methods was evaluated by the following statistical tests: ‘Kappa’, ‘The adjusted rand’, McNemar´s test for marginal symmetry, Proportion of agreement P , P , P , and Odds Ratio. The highest agreement was found between the 0 + - 2 types of sock sampling, while the lowest agreement was found by comparing 60 fae- cal samples with 5 pairs of socks. Two pairs of socks analysed as one pool appeared to be just as effective in detecting S. enterica as the 60 faecal samples. In broiler flocks, 5 pairs of socks were used both in the routine samples taken at about 3 weeks of age for the establishment o