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conservation and diversity of gene families explored using the codehop strategy in higher plants保护和基因家族的多样性研究在高等植物中使用codehop策略.pdf

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conservation and diversity of gene families explored using the codehop strategy in higher plants保护和基因家族的多样性研究在高等植物中使用codehop策略

BMC Plant Biology BioMed Central BMC Plant Biology x 2002, 2 Methodology article Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants Marc Morant, Alain Hehn and Danièle Werck-Reichhart* Address: Department of Plant Stress Response, Institute of Plant Molecular Biology, CNRS-UPR 2357, Université Louis Pasteur, 28 rue Goethe, 67083 Strasbourg, France E-mail: Marc Morant - marc.morant@ibmp-ulp.u-strasbg.fr; Alain Hehn - alain.hehn@ibmp-ulp.u-strabg.fr; Danièle Werck- Reichhart* - daniele.werck@ibmp-ulp.u-strasbg.fr *Corresponding author Published: 1 August 2002 Received: 24 April 2002 Accepted: 1 August 2002 BMC Plant Biology 2002, 2:7 This article is available from: /1471-2229/2/7 © 2002 Morant et al; licensee BioMed Central Ltd. This article is published in Open Access: verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Availability of genomewide information on an increasing but still limited number of plants offers the possibility of identifying orthologues, or related genes, in species with major economical impact and complex genomes. In this paper we exploit the recently described CODEHOP primer design and PCR strategy for targeted isolation of homologues in large gene families. Results: The method was tested with two different objectives. The first was to analy