composition and functional analysis of low-molecular-weight glutenin alleles with aroona near-isogenic lines of bread wheat低分子量麦谷蛋白的组成和功能分析等位基因与aroona near-isogenic行面包小麦.pdfVIP
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composition and functional analysis of low-molecular-weight glutenin alleles with aroona near-isogenic lines of bread wheat低分子量麦谷蛋白的组成和功能分析等位基因与aroona near-isogenic行面包小麦
Zhang et al. BMC Plant Biology 2012, 12:243
/1471-2229/12/243
RESEARCH ARTICLE Open Access
Composition and functional analysis of
low-molecular-weight glutenin alleles with
Aroona near-isogenic lines of bread wheat
1,2† 1† 1 2 3 1 1,4*
Xiaofei Zhang , Hui Jin , Yan Zhang , Dongcheng Liu , Genying Li , Xianchun Xia , Zhonghu He
and Aimin Zhang2*
Abstract
Background: Low-molecular-weight glutenin subunits (LMW-GS) strongly influence the bread-making quality of
bread wheat. These proteins are encoded by a multi-gene family located at the Glu-A3, Glu-B3 and Glu-D3 loci on
the short arms of homoeologous group 1 chromosomes, and show high allelic variation. To characterize the
genetic and protein compositions of LMW-GS alleles, we investigated 16 Aroona near-isogenic lines (NILs) using
SDS, 2Dand the LMW-GS gene marker system. Moreover, the composition of glutenin macro-polymers,
dough properties and pan bread quality parameters were determined for functional analysis of LMW-GS alleles in
the NILs.
Results: Using the LMW-GS gene marker system, 14–20 LMW-GS genes were identified in individual NILs. At the
Glu-A3 locus, two m-type and 2–4 i-type genes were identified and their allelic variants showed high
polymorphisms in length and nucleotide sequences. The Glu-A3d allele possessed three active genes, the highest
number among Glu-A3 alleles. At the Glu-B3 locus, 2–3 m-type and 1–3 s-type genes were identified from individual
NILs. Based on the different compositions of s-type genes, Glu-B3 alleles were divided into two groups, one
containing Glu-B3a, B3b, B3f and B3g, and the other comprising Glu-B3c, B3d, B3h and B3i. Eight conserved genes
were identified among Glu-D3 alleles, except for Glu
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