可诱导性共刺激分子（ICOS）单抗对T细胞诱导胰岛细胞凋亡及胰岛功能影响的研究.pdfVIP

i#京{《f：}人学坝lj号’{讧谤支 TheEffectofICOS onthe andFunction AntibodyApoptosis ofTcell-InducedIsletCells ABSTRACT theeffectofthe1COS onthe Objective：Toinvestigate antibody and ofTcell cells function inducedislet anditsmechanisms． apoptosis Methods：TheRINm5feeliandratT were cells CO—culturedwithICOS for1 effectofICOS on antibody2h、24h、48h．(1)The protective antibody the ofRINm5fcell T straininducedcellswasobserved apoptosis by by flow theendof cellularRNAwas 48h，total cytometry．(2)At extracted， andthemRNAofthe and BAX、Bcl-2the ofthe genes apoptoticpathway， such RT-PCR effectofthe on method．(3)The ICOS theislet by antibody insulin INS1、INS2andsome factorswas genes detected transcription by RT-PCRmethod cellincreasedthe rateofislet Results：(1)T apoptotic cell．After CO—culturedwithICOS rateof cells antibody，theapoptotictheRINm5f decreased andinterferon could the