洪永聪-氯氰菊酯降解菌T-1的诱变育种及其产酶条件研究-090507.docVIP

氯氰菊酯降解菌T-1的诱变育种及其产酶条件研究 洪永聪1 王军强2 辛伟3 纪国才2 崔德杰3* （1青岛农业大学生命科学学院，山东 青岛 266109；2青岛市农业技术推广站；3青岛农业大学资源与环境学院） 摘要：以从菜园土壤内分离到对氯氰菊酯降解率较高的菌株T1（Paenibacillus alginolyticus）为出发菌，进行化学诱变和紫外诱变。结果显示，DES对菌株的最佳诱变条件为3% 60min、4% 40min5% 20min，紫外线对对菌株的最佳诱变条件为cm 10min或15cm 20min。通过筛选，获得株具有一定的遗传稳定性。KB）、培养温度（40℃）、底物浓度(50mg/L)、培养时间（96h）、初始pH（6.6）和装液量(25ml/250ml)。 关键词：降解菌；解藻朊类芽孢杆菌 中图分类号：Q965.8 文献标识码：A Selection by utation of cypermethrin degrading strain T-1 and its optimal conditions of producing enzyme HONG Yong-cong1 WANG Jun-qiang2 XIN Wei3 JI Guo-cai2 CUI De-jie3* (1 College of Life Science, Qingdao Agriculture University, Qingdao, 266109 China; 2 Qingdao Station for Popularizing Agricultural Technique; 3 College of Resource and Environment, Qingdao Agriculture University) Abstract: Cypermethrin degrading strain T-1 (Paenibacillus alginolyticus) was used as the original strain, which was isolated from garden soil. The result indicated that its optimal condition induced by DES should be 3% 60min, 4% 40min and 5% 20min. The optimal condition of strain T-1 induced by UV light was tested to be 5cm 8min, 10cm 10min and 15cm 20min. Nine mutants were obtained, among which five mutants were proved to degrade cypermethrin on a higher level, compared with their original strain. One of those mutants were tested to have hereditary stability to degrade cypermethrin. The optimum conditions of producing cypermethrin degrading enzyme was tested to be medium (KB), cultivation temperature (40℃), substrate concentration (50mg/L), cultivation time (96h), original pH (6.6) and liquid medium volume (25ml/250ml). Key words: Cypermethrin degrading bacteria; Paenibacillus alginolyticus; mutation breeding; the optimal conditions of producing enzyme 收稿日期： 基金项目：国家自然科学基金资助项目（No；山东省教育厅科技计划项目（No.J06K02）。 作者简介：洪永聪（1977-），男，博士，讲师，主要研究方向生物防治。 拟除虫菊酯类农药在叶菜类蔬菜中农药残留普遍且危害性比较大[1]。微生物是农药转化的重要因素之一，生物修复也己被广泛地应用于降解环境中的有毒成分，并日益引起人们的重视。迄今为止，各国研究人员已从土壤、污泥、污水、天然水体、垃圾场和厩肥中分离到降解不同农药的活性微生物[2-4]。国内外很多有关农药残留降解菌研究的报道，但对氯氰菊酯类