总氮的测定碱性过硫酸钾消解紫外分光光度法-食品检验检测（Determination of total nitrogen alkaline potassium persulfate digestion UV spectrophotometry - Food Inspection）.docVIP

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总氮的测定碱性过硫酸钾消解紫外分光光度法-食品检验检测（Determination of total nitrogen alkaline potassium persulfate digestion UV spectrophotometry - Food Inspection）.doc

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总氮的测定碱性过硫酸钾消解紫外分光光度法-食品检验检测（Determination of total nitrogen alkaline potassium persulfate digestion UV spectrophotometry - Food Inspection）

总氮的测定碱性过硫酸钾消解紫外分光光度法-食品检验检测（Determination of total nitrogen alkaline potassium persulfate digestion UV spectrophotometry - Food Inspection） Determination of total nitrogen: alkaline potassium persulfate digestion UV spectrophotometric method 2.1 filtration total nitrogen: the amount of nitrogen containing soluble and filtered solids (less than 0.45 m particles) in water. 2.2 total nitrogen: the amount of nitrogen in soluble and suspended particles. 3 principle In 60 DEG C water solution, potassium persulfate and potassium bisulfate can be decomposed to produce atomic oxygen, potassium bisulfate dissociate in solution and produce hydrogen ions, so in alkaline medium of sodium hydroxide can accelerate the decomposition process tends to be completely. The decomposed atomic oxygen can be converted into nitrate at the temperature of 120~124 degrees. And in the process, the organic compounds are simultaneously oxidized and decomposed. The absorbance A220 and A275 can be measured at 220 and 275nm wavelength respectively by ultraviolet spectrophotometry. The corrected absorbance A is determined by formula (1): A = A2202A275...................................................... (1) Check the calibration curve according to the value of A and calculate the total nitrogen content (NO3 N meter). 4 reagents and materials Unless otherwise specified in (4.1), analytical reagents are in accordance with national or professional standards for analysis. 4.1 water, no ammonia. Prepared by one of the following methods; 4.1.1 ion exchange method: distilled water is passed through a strong acid cation exchange resin (hydrogen) column, and the effluent is collected in a glass bottle with a sealed glass cover. 4.1.2 Distillation: in 1000mL distilled water, add 0.10mL sulfuric acid (p=1.84g / mL). In a full glass distiller, the 50mL is distilled and the effluent is removed, and the distillate is collected in a glass bottle with a glass stopper. 4.2 sodium hydroxide solution, 200g / L: take 20