含环氧基固定化葡萄糖氧化酶共聚载体的催化性能研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.12 含环氧基固定化葡萄糖氧化酶共聚载体的 催化性能研究 李丕武，李瑞瑞，刘佃磊，郝琼，杨晴，王升 （齐鲁工业大学食品与生物工程学院，山东济南 250353） 摘要：环氧基团可以在温和条件下与酶分子的氨基发生共价结合使其固定于载体表面。选用含有活性环氧基团的甲基丙烯酸缩 水甘油酯(GMA)为单体，N,N´-亚甲基双丙烯酰胺(MBAA)为交联剂，聚乙烯吡咯烷酮(PVP)为稳定剂，2,2´-偶氮二异丁腈(AIBN)为引 发剂,乙醇水溶液为分散介质，并加入Fe O 磁流体,通过反相悬浮聚合成功合成了大孔Ferrofluid-GMA-MBAA 共聚物载体(FGM) 。 3 4 通过调节磁流体和交联剂用量,可调节载体比表面积,孔径以及溶胀性能。将葡萄糖氧化酶(GOD)偶联于GMA 含量20% ，磁流体含量 4% ，交联剂含量40%的共聚物载体4FGM (40)，制成固定化葡萄糖氧化酶,其表观酶活高达546.23±2.33 U/g。讨论了固定化葡萄糖氧 化酶的酶学性质，在最适条件(55 ℃，pH 8.0)下，固定化酶的表观酶活回收率为90.45%；连续使用15 次活性仍接近初始值的60%； 固定化酶在4 ℃保存30 d，活性保持不变。 关键词：环氧基；反相悬浮聚合; 固定化酶；葡萄糖氧化酶 文章篇号：1673-9078(2013)12-2926-2930 Properties of the Hydrophilic Copolymer Supporter Containing Oxirane for Immobilized Glucose Oxidase LI Pi-wu, LI Rui-rui, LIU Dian-lei, HAO Qiong, YANG Qing, WANG Sheng (School of Food and Bioengineering, Qilu University of Technology, Jinan 250353, China) Abstract: Oxirane groups can react with the amino groups of enzyme under mild conditions so that could be immobilized on the sur face of copolymer supporter. The macroporous Ferrofluid-GMA-MBAA (FGM) polymer were synthesized by inverse suspension polymerization with glycidyl methacrylate (GMA) as the reactive monomer and N ,N′-methylene-bis-acrylamide (MBAA) as the cross-linking agent. Polyvinylpyrrolidone (PVP) was used as the stabilizer monomer and azodiisobutyronitrile (AIBN) was used as the initiator monomer. Fe O was 3 4 added as magnetofluid to the dispersion medium, ethanol solution. The effects of the amount of magnetofluid and crossli