a communal bacterial adhesin anchors biofilm and bystander cells to surfaces公共细菌adhesin锚生物膜和旁观者细胞表面.pdfVIP

A Communal Bacterial Adhesin Anchors Biofilm and Bystander Cells to Surfaces Cedric Absalon, Katrina Van Dellen, Paula I. Watnick* Division of Infectious Disease, Children’s Hospital, Boston, Massachusetts, United States of America Abstract While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm. Citation: Absalon C, Van Dellen K, Watnick PI (2011) A Communal Bacterial Adhesin Anchors Biofilm and Bystander Cells to Surfaces. PLoS Pathog 7(8): e1002210. doi:10.1371/journal.ppat.1002210 Editor: Ralph R. Isberg, Tufts University School of Medicine, United States of America Received January 17, 2011; Accepted June 26, 2011; Published August 25, 2011 Copyright: 2011 Absalon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided th