a comparative study of impedance versus optical label-free systems relative to labelled assays in a predominantly gi coupled gpcr (c5ar) signalling比较研究的阻抗与光学label-free系统相对于标签检测主要在胃肠道耦合gpcr信号(c5ar).pdfVIP

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a comparative study of impedance versus optical label-free systems relative to labelled assays in a predominantly gi coupled gpcr (c5ar) signalling比较研究的阻抗与光学label-free系统相对于标签检测主要在胃肠道耦合gpcr信号(c5ar).pdf

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a comparative study of impedance versus optical label-free systems relative to labelled assays in a predominantly gi coupled gpcr (c5ar) signalling比较研究的阻抗与光学label-free系统相对于标签检测主要在胃肠道耦合gpcr信号(c5ar)

Biosensors 2012, 2, 273-290; doi:10.3390/bios2030273 OPEN ACCESS biosensors ISSN 2079-6374 /journal/biosensors/ Article A Comparative Study of Impedance versus Optical Label-Free Systems Relative to Labelled Assays in a Predominantly Gi Coupled GPCR (C5aR) Signalling Reena Halai, Daniel E. Croker, Jacky Y. Suen, David P. Fairlie and Matthew A. Cooper * Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia; E-Mails: r.halai@.au (R.H.); d.croker@.au (D.E.C.); j.suen@.au (J.Y.S.); d.fairlie@.au (D.P.F.) * Author to whom correspondence should be addressed; E-Mail: er@.au; Tel.: +61-7-3346-2044; Fax: +61-7-3346-2101. Received: 30 May 2012; in revised form: 11 July 2012 / Accepted: 24 July 2012 / Published: 26 July 2012 Abstract: Profiling ligand function on G-protein coupled receptors (GPCRs) typically involves using transfected cells over-expressing a target of interest, a labelled ligand, and intracellular secondary messenger reporters. In contrast, label-free assays are sensitive enough to allow detection in native cells, which may provide a more physiologically relevant readout. Here, we compare four agonists (native agonists, a peptide full agonist and a peptide partial agonist) that stimulate the human inflammatory GPCR C5aR. The receptor was challenged when present in human monocyte-derived macrophages (HMDM) versus stably transfected human C5aR-CHO cells. Receptor activation was compared on label