a de novo case of floating chromosomal polymorphisms by translocation in quasipaa boulengeri (anura, dicroglossidae)新创的浮动的易位染色体多态性quasipaa boulengeri(无尾目动物,dicroglossidae).pdfVIP

A De Novo Case of Floating Chromosomal Polymorphisms by Translocation in Quasipaa boulengeri (Anura, Dicroglossidae) 1,2 1,2 1 1 Liyan Qing , Yun Xia , Yuchi Zheng , Xiaomao Zeng * 1 Department of Herpetology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, People’s Republic of China, 2 Graduate University of Chinese Academy of Sciences, Beijing, People’s Republic of China Abstract Very few natural polymorphisms involving interchromosomal reciprocal translocations are known in amphibians even in vertebrates. In this study, thirty three populations, including 471 individuals of the spiny frog Quasipaa boulengeri, were karyotypically examined using Giemsa stain or FISH. Five different karyomorphs were observed. The observed heteromorphism was autosomal but not sex-related, as the same heteromorphic chromosomes were found both in males and females. Our results indicated that the variant karyotypes resulted from a mutual interchange occurring between chromosomes 1 and 6. The occurrence of a nearly whole-arm translocation between chromosome no. 1 and no. 6 gave rise to a high frequency of alternate segregation and probably resulted in the maintenance of the translocation polymorphisms in a few populations. The translocation polymorphism is explained by different frequencies of segregation modes of the translocation heterozygote during meiosis. Theoretically, nine karyomorphs should be investigated, however, four expected karyotypes were not found. The absent karyomorphs may result from recessive lethal mutations, position effects, duplications and deficiencies. The phylogenetic inference proved that all populations of Q. boulengeri grouped into a