a dna vaccine encoding multiple hiv cd4 epitopes elicits vigorous polyfunctional, long-lived cd4+ and cd8+ t cell responses艾滋病毒dna疫苗编码多个cd4抗原表位引发激烈的多重的,长寿的cd4 +和cd8 + t细胞反应.pdfVIP

A DNA Vaccine Encoding Multiple HIV CD4 Epitopes Elicits Vigorous Polyfunctional, Long-Lived CD4+ and CD8+ T Cell Responses 1,3,4. 1,3. 1 2,3 Daniela Santoro Rosa , Susan Pereira Ribeiro , Rafael Ribeiro Almeida , Eliane Conti Mairena , ´ 2,3, Jorge Kalil1,2,3, Edecio Cunha-Neto1,2,3* Edilberto Postol ˜ 1 Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of Sao Paulo School of Medicine, ˜ ˜ ˜ ˜ Sao Paulo, Brazil, 2 Heart Institute (InCor), University of Sao Paulo School of Medicine, Sao Paulo, Brazil, 3 Institute for Investigation in Immunology-INCT, Sao Paulo, Brazil, ˜ ˜ 4 Division of Immunology-Federal University of Sao Paulo-UNIFESP, Sao Paulo, Brazil Abstract T-cell based vaccines against HIV have the goal of limiting both transmission and disease progression by inducing broad and functionally relevant T cell responses. Moreover, polyfunctional and long-lived specific memory T cells have been associated to vaccine-induced protection. CD4+ T cells are important for the generation and maintenance of functional CD8+ cytotoxic T cells. We have recently developed a DNA vaccine encoding 18 conserved multiple HLA-DR-binding HIV-1 CD4 epitopes (HIVBr18), capable of