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a molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep分子诊断工具来代替幼虫文化传统粪便蛋数减少测试的羊
A Molecular Diagnostic Tool to Replace Larval Culture in
Conventional Faecal Egg Count Reduction Testing in
Sheep
1 2 2 1,2 1
Florian Roeber *, John W. A. Larsen , Norman Anderson , Angus J. D. Campbell , Garry A. Anderson ,
Robin B. Gasser1*, Aaron R. Jex1
1 Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia, 2 Mackinnon Project, The University of Melbourne, Werribee, Victoria, Australia
Abstract
The accurate diagnosis of parasitic nematode infections in livestock (including sheep and goats) is central to their effective
control and the detection of the anthelmintic resistance. Traditionally, the faecal egg count reduction test (FECRT),
combined with the technique of larval culture (LC), has been used widely to assess drug-susceptibility/resistance in
strongylid nematodes. However, this approach suffers from a lack of specificity, sensitivity and reliability, and is time-
consuming and costly to conduct. Here, we critically assessed a specific PCR assay to support FECRT, in a well-controlled
experiment on sheep with naturally acquired strongylid infections known to be resistant to benzimidazoles. We showed
that the PCR results were in close agreement with those of total worm count (TWC), but not of LC. Importantly, albendazole
resistance detected by PCR-coupled FECRT was unequivocally linked to Teladorsagia circumcincta and, to lesser extent,
Trichostrongylus colubriformis, a result that was not achievable by LC. The key findings from this study demonstrate that our
PCR-coupled FECRT approach has major merit for supporting anthelmintic resistance in nematode populat
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