a deeply branching thermophilic bacterium with an ancient acetyl-coa pathway dominates a subsurface ecosystem深深分支嗜热细菌与古代乙酰辅酶a通路主导地下生态系统.pdfVIP

A Deeply Branching Thermophilic Bacterium with an Ancient Acetyl-CoA Pathway Dominates a Subsurface Ecosystem 1 2 1 3 4 1 Hideto Takami *, Hideki Noguchi , Yoshihiro Takaki , Ikuo Uchiyama , Atsushi Toyoda , Shinro Nishi , 1 1 5 2 6 5 Gab-Joo Chee , Wataru Arai , Takuro Nunoura , Takehiko Itoh , Masahira Hattori , Ken Takai 1 Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan, 2 Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan, 3 Laboratory of Genome Informatics, National Institute for Basic Biology, National Institutes of Natural Sciences, Aichi, Japan, 4 National Institute of Genetics, Mishima, Japan, 5 Subground Animalcule Retrieval (Sugar) Project, Natsushima, Japan, 6 Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan Abstract A nearly complete genome sequence of Candidatus ‘Acetothermum autotrophicum’, a presently uncultivated bacterium in candidate division OP1, was revealed by metagenomic analysis of a subsurface thermophilic microbial mat community. Phylogenetic analysis based on the concatenated sequences of proteins common among 367 prokaryotes suggests that Ca. ‘A. autotrophicum’ is one of the earliest diverging bacterial lineages. It possesses a folate-dependent Wood-Ljungdahl (acetyl-CoA) pathway of CO2 fixation, is predicted to have an acetogenic lifestyle, and possesses the newly discovered archaeal-autotrophic type of bifunctional fructose 1,6-bisphosphate aldolase/phosphatase. A phylogenetic