a novel method for the preparation of retinoic acid-loaded nanoparticles一种新型载有维甲酸的制备纳米粒子的方法.pdfVIP

Int. J. Mol. Sci. 2009, 10, 2336-2347; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A Novel Method for the Preparation of Retinoic Acid-Loaded Nanoparticles Cesare Errico, Matteo Gazzarri and Federica Chiellini * Laboratory of Bioactive Polymeric Materials for Biomedical Environmental Applications (BIOlab), UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Vecchia Livornese 1291, 56122 S. Piero a Grado, Pisa, Italy; E-Mails: cesare@ns.dcci.unipi.it (C.E.); m.gazzarri@ns.dcci.unipi (M.G.) * Author to whom correspondence should be addressed; E-Mail: federica@dcci.unipi.it; Tel. +39 050 2210305; Fax: +39 050 2210332 Received: 27 April 2009; in revised form: 14 May 2009 / Accepted: 18 May 2009 / Published: 19 May 2009 Abstract: The goal of present work was to investigate the use of bioerodible polymeric nanoparticles as carriers of retinoic acid (RA), which is known to induce differentiation of several cell lines into neurons. A novel method, named “Colloidal-Coating”, has been developed for the preparation of nanoparticles based on a copolymer of maleic anhydride and butyl vinyl ether (VAM41) loaded with RA. Nanoparticles with an average diameter size of 70 nm and good morphology were prepared. The activity of the encapsulated RA was evaluated on SK-N-SH human neuroblastoma cells, which are known to undergo