altered synaptic properties during integration of adult-born hippocampal neurons following a seizure insult改变adult-born海马神经元的突触属性在集成后发作的侮辱.pdfVIP

Altered Synaptic Properties During Integration of Adult- Born Hippocampal Neurons Following a Seizure Insult 1,2¤ 1,2 1,2 1,2 ¨ 1,2 1,2 Johanna Jackson , Deepti Chugh , Per Nilsson , James Wood , Karl Carlstrom , Olle Lindvall , Christine T. Ekdahl1,2,3* 1 Laboratory of Neurogenesis and Cell Therapy, Wallenberg Neuroscience Center, Lund, Sweden, 2 Lund Stem Cell Center, Lund University, Lund, Sweden, 3 Inflammation and Stem Cell Therapy Group, Division of Clinical Neurophysiology, Lund, Sweden Abstract Pathological conditions affect several stages of neurogenesis in the adult brain, including proliferation, survival, cell fate, migration, and functional integration. Here we explored how a pathological environment modulates the heterogeneous afferent synaptic input that shapes the functional properties of newly formed neurons. We analyzed the expression of adhesion molecules and other synaptic proteins on adult-born hippocampal neurons formed after electrically-induced partial status epilepticus (pSE). New cells were labeled with a GFP-retroviral vector one week after pSE. One and three weeks thereafter, synaptic proteins were present on dendritic spines and shafts, but without differences between pSE and control group. In contrast, at six weeks, we found fewer dendritic spines and decreased expression of the scaffolding protein PSD-95 on spines, without changes in expression of the adhesion molecules N-cadherin or neuroligin-1, primarily located at excitatory synapses. Moreover, we detected an increased expression of the inhibitory scaffolding protein gephyrin in newborn but not mature neurons after SE. However, this incre