antibodies targeting the pfrh1 binding domain inhibit invasion of plasmodium falciparum merozoites抗体针对pfrh1绑定域抑制入侵恶性疟原虫裂殖子.pdfVIP

Antibodies Targeting the PfRH1 Binding Domain Inhibit Invasion of Plasmodium falciparum Merozoites 1 1 1 1 1 1 Xiaohong Gao , Kim Pin Yeo , Siqi Sharon Aw , Claudia Kuss , Jayasree K. Iyer , Saraswathy Genesan , 2 2 1 1 Ravikumar Rajamanonmani , Julien Lescar , Zbynek Bozdech , Peter R. Preiser * 1 Division of Genomics Genetics, School of Biological Sciences, Nanyang Technological University, Singapore, 2 Division of Structural Computational Biology, School of Biological Sciences, Nanyang Technological University, Singapore Abstract Invasion by the malaria merozoite depends on recognition of specific erythrocyte surface receptors by parasite ligands. Plasmodium falciparum uses multiple ligands, including at least two gene families, reticulocyte binding protein homologues (RBLs) and erythrocyte binding proteins/ligands (EBLs). The combination of different RBLs and EBLs expressed in a merozoite defines the invasion pathway utilized and could also play a role in parasite virulence. The binding regions of EBLs lie in a conserved cysteine-rich domain while the binding domain of RBL is still not well characterized. Here, we identify the erythrocyte binding region of the P. falciparum reticulocyte binding protein homologue 1 (PfRH1) and show that antibodies raised against the functional binding region efficiently inhibit invasion. In addition, we directly demonstrate that changes in the expression of RBLs can constitute an immune evasion mechanism of the malaria merozoite. Citation: Gao X, Yeo KP, Aw SS, Kuss C, Iyer JK, et al. (2008) Antibodies Targeting the P