an optimized protocol for dna extraction from wheat seeds and loop-mediated isothermal ampli？cation (lamp) to detect fusarium graminearum contamination of wheat grain一个优化协议dna提取小麦种子和loop-mediated等温ampli 花药对赤霉病阳离子(灯)检测小麦粮食的污染.pdfVIP

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an optimized protocol for dna extraction from wheat seeds and loop-mediated isothermal ampli？cation (lamp) to detect fusarium graminearum contamination of wheat grain一个优化协议dna提取小麦种子和loop-mediated等温ampli 花药对赤霉病阳离子(灯)检测小麦粮食的污染.pdf

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an optimized protocol for dna extraction from wheat seeds and loop-mediated isothermal ampli？cation (lamp) to detect fusarium graminearum contamination of wheat grain一个优化协议dna提取小麦种子和loop-mediated等温ampli 花药对赤霉病阳离子(灯)检测小麦粮食的污染

Int. J. Mol. Sci. 2011, 12, 3459-3472; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article An Optimized Protocol for DNA Extraction from Wheat Seeds and Loop-Mediated Isothermal Ampliﬁcation (LAMP) to Detect Fusarium graminearum Contamination of Wheat Grain Kamel Abd-Elsalam 1,2,3,*, Ali Bahkali 1,2, Mohamed Moslem 1, Osama E. Amin 1 and 4 Ludwig Niessen 1 King Saud University, College of Science, Botany and Microbiology Department, P.O. Box 2455, Riyadh 1145, Saudi Arabia; E-Mails: abhakali@.sa (A.B.); mbmoslem@.sa (M.M.); osamaemam@ (O.A.) 2 Center of Excellence in Biotechnology Research, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia 3 Plant Pathology Research Institute, Agricultural Research Center, Giza 12655, Egypt 4 Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising, Germany; E-Mail: niessen@wzw.tum.de * Author to whom correspondence should be addressed; E-Mail: kamel200@.sa; Tel.: +966-92-1467-5870; Fax: +966-1467-5833. Received: 31 March 2011; in revised form: 28 April 2011 / Accepted: 18 May 2011 / Published: 1 June 2011 Abstract: A simple, rapid, and efficient method for isolating genomic DNA from germinated seeds of wheat that is free from polysaccharides and polyphenols is reported. DNA was extracted, treated with RNase, measured and tested for completeness us